Instructions: Click on a dye vial to load the pipette, then click near the pencil line on the paper to place a spot.

Paper placed in sealed chamber with water solvent.

Instructions: Drag the ruler and align its 0 cm mark with the pencil line (origin). Measure from the origin to the solvent front and to the center of each spot. Record your measurements in the table below. Calculate and enter the Rf values using the formula: Rf = (distance traveled by spot) / (distance traveled by solvent front)